Document Type
Thesis
Date of Award
12-31-1988
Degree Name
Master of Science in Biomedical Engineering - (M.S.)
Department
Biomedical Engineering Committee
First Advisor
Arthur B. Ritter
Second Advisor
David S. Kristol
Third Advisor
Piero M. Armenante
Abstract
It has long been known that blood - tissue transport of macromolecules in the microcirculation is driven by diffusion. Although two dimensional models fit the experimental data well, the diffusion process is three dimensional.The objective of this research was to establish a three dimensional unsteady state mathematical model for intravascular transport of macromolecules, and suppoi t the theoretical establishment by experimental results.
Three dimensional diffusion of macromolecules through the interstitial space in the hamster cheek pouch was studied, with intravital fluorescence microscopy. After application of bradykinin, the movement of Fluorescein isothiocynate (F.I.T.C.) - labeled Dextrans of average molecular weight 150,000 daltons ( Dextran 150) was recorded on video tape. Video tapes were subsequently played back frame by frame, and analyzed by digital image processing. The digital image analysis was carried out using QUANTEX frame grabber - digitizer and software IMAGE, developed in our laboratory.
Observations, both in our laboratory and by other investigators, have observed that blood-tissue transport of macromolecules occurs primarily at discreet leakage sites located at post - capillary venules of 10 - 25 /cm in diameteis. In addition, it is believed that transport of macromoleculginvolves both diffusion and convective mechanisms. Therefore a three dimensional mathematical model Diffusion from a Point Source in a Constant velocity Stream was adapted to simulate macromolecular transport. The results of model simulation were compared with experimental data. The parameters required for the simulation ( Velocities and Diffusion Coefficient) were estimated from the literature.
Recommended Citation
Khimani, Nikhil V., "Three dimensional study of macromolecular leakage patterns using video image analysis" (1988). Theses. 3117.
https://digitalcommons.njit.edu/theses/3117
