Functional clotting assay to monitor low molecular weight heparin dosage

Document Type

Conference Proceeding

Publication Date

1-1-2002

Abstract

Low molecular weight heparin (LMWH), an anticoagulant drug, has seen increased use in a variety of clinical situations. Mercuric ion (Hg2+) has been shown to increase procoagulant activity via Tissue Factor (TF) stimulated production of Factor Xa, a pathway that has been linked to many thrombotic disorders. The effects of Hg2+ on heparinized blood may provide valuable insight into the effectiveness of LMWH to counteract TF-induced hypercoagulability. We performed in vitro studies by spiking human citrated whole blood (CWB) with HgCl2 and LMWH at final concentrations of 0.01% HgCl2, 0.5 U/mL LMWH, and a combination of these agents. The samples (n=19) were incubated at 37° for 10 minutes and recalcified with 0.1 M CaCl2 to initiate clotting. The clotting or recalcification time (RT) was measured with a Sonoclot Coagulation Analyzer. The results indicate that HgCl2 was able to significantly (p<0.0001) reduce the anticoagulant ability of LMWH. The data warrants a reassessment of current LMWH monitoring and therapeutic protocols.

Identifier

0041835990 (Scopus)

Publication Title

Proceedings of the IEEE Annual Northeast Bioengineering Conference Nebec

e-ISSN

21607001

ISSN

1071121X

First Page

45

Last Page

46

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