Date of Award
Master of Science in Chemical Engineering - (M.S.)
Chemical Engineering, Chemistry and Environmental Science
Piero M. Armenante
Kinetic experiments were conducted using Pseudomonas putida (ATCC 17484) to determine the rate of growth when naphthalene was provided as a sole carbon source in suspended biomass systems. Batch and chemostat reactors were used under three sets of conditions: non-aerated, aerated at 29.5° C, and aerated at 25.5° C. A number of concentrations were tested under each set of conditions. Data regression was used to determine parameters for Monod (non-inhibitory), Andrews (inhibitory), and zero-order kinetics. Although the Andrews fit of the data provided a slightly lower sum-of-squares error (SSE), the amount of data scatter and the weak inhibition made the Andrews fit only marginally better. In addition, extremely small values of the saturation constant (Ks < 0.01mg/L) made the use of a zero-order kinetic constant nearly identical with a Monod model for naphthalene concentrations above 0.05 mg/L. Particularly troublesome experimental problems included inconsistent biomass measurements by optical density and wall growth. Suggested biokinetic parameters are: µ = 0.0074 min-1, Ks= 0.0022 mg/L, and K1= 31 mg/L (for Andrews model); µmax =0.0057 min-1, and Ks= 0.00088 mg/L (for Monod model); and k0= 0.0058 min-1 (for the zero-order model).
Best, Jay Boyd, "Biodegradation of naphthalene using Pseudomonas putida (ATCC 17484) in batch and chemostat reactors" (1997). Theses. 996.