Document Type
Thesis
Date of Award
5-31-1989
Degree Name
Master of Science in Biomedical Engineering - (M.S.)
Department
Biomedical Engineering Committee
First Advisor
Clarence W. Mayott
Second Advisor
J. Russell Parsons
Third Advisor
David S. Kristol
Abstract
A composite bone graft material which can induce or promote new bone growth has potential orthopaedic applications in the repair of bone defects or the stabilization of surgically implanted devices. This material should be biocompatible, have good handling properties, promote osteoinduction and/or osteoconduction and have a good shelf life. Demineralized bone matrix and bone morphogenetic protein have been demonstrated to promote osteoinduction in vitro and in vivo.
This in vitro study examined the inductive ability of an osteoinductive protein, bone morphogenetic protein, when absorbed on non-active devitalized bone matrix. In culture, fetal rat muscle was grown on powdered rabbit demineralized bone matrix (positive control), devitalized bone matrix (negative control) and devitalized bone matrix plus bone morphogenetic protein to determine the differentiation of the mesenchymal cells, chondrogenesis. This modified tissue culture model showed chondrogenesis of the cells grown on the demineralized bone matrix, no chondrogenesis with devitalized bone matrix and unexpectedly, no enhancement of the chondrogeneis with the osteoinductive factor added to the devitalized bone matrix.
Recommended Citation
Alexander, Levelle Elise Burr, "In vitro biocompatibility study to determine the synergistic effect of components for an artificial bone graft material" (1989). Theses. 2704.
https://digitalcommons.njit.edu/theses/2704
