Date of Award

Spring 2015

Document Type

Thesis

Degree Name

Master of Science in Biomedical Engineering - (M.S.)

Department

Biomedical Engineering

First Advisor

Eun Jung Lee

Second Advisor

Treena Livingston Arinzeh

Third Advisor

Cheul H. Cho

Abstract

Embryonic stem cells have the potential to differentiate into multiple cell types including insulin-producing cells (IPCs), which is becoming one of the promising cell sources for treating type 1 diabetes mellitus. However, in order to achieve functional stem cell-derived cells, it is important to generate more mature IPCs and to keep long-term viability post differentiation process. In this study, we varied several factors including different embryonic body culture conditions, digested cells seeding density and various coatings required for differentiation to optimize a previously established protocol to enhance the overall differentiation efficiency. Moreover, a three-dimensional in vitro collagen tissue culture system was prepared to provide a more physiological culture environment for stem cell-derived IPCs. Survivability of IPCs was examined under both static and flow conditions and low flow rate of 0.02 ml/min resulted in better survival of IPCs in in vitro three-dimensional tissues.

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