Document Type
Thesis
Date of Award
Spring 5-31-2015
Degree Name
Master of Science in Biomedical Engineering - (M.S.)
Department
Biomedical Engineering
First Advisor
Eun Jung Lee
Second Advisor
Treena Livingston Arinzeh
Third Advisor
Cheul H. Cho
Abstract
Embryonic stem cells have the potential to differentiate into multiple cell types including insulin-producing cells (IPCs), which is becoming one of the promising cell sources for treating type 1 diabetes mellitus. However, in order to achieve functional stem cell-derived cells, it is important to generate more mature IPCs and to keep long-term viability post differentiation process. In this study, we varied several factors including different embryonic body culture conditions, digested cells seeding density and various coatings required for differentiation to optimize a previously established protocol to enhance the overall differentiation efficiency. Moreover, a three-dimensional in vitro collagen tissue culture system was prepared to provide a more physiological culture environment for stem cell-derived IPCs. Survivability of IPCs was examined under both static and flow conditions and low flow rate of 0.02 ml/min resulted in better survival of IPCs in in vitro three-dimensional tissues.
Recommended Citation
Ma, Xiaotang, "Differentiation of mouse embryonic stem cells into insulin-producing cells: optimization to enhance the overall differentiation efficiency" (2015). Theses. 235.
https://digitalcommons.njit.edu/theses/235
