Document Type


Date of Award


Degree Name

Master of Science in Chemical Engineering - (M.S.)


Chemical Engineering and Chemistry

First Advisor

Hung T. Chen

Second Advisor

Deran Hanesian

Third Advisor

Edward Charles Roche, Jr.


A pH parametric pump, coupled with ion exchange, was used in an experimental investigation to separate a protein-water binary mixture. The model system consisted of a hemoglobin-water binary mixture. A pH gradient throughout the parametric pumping column was sustained with the use of hollow fiber devices incorporating phosphate buffer circulation.

Data is presented for both batch and semi-continuous recuperative mode pH parametric pumping. Continuous pH parametric pumping for protein isolation has also been envisioned.

No attempt has been made to optimize this newly devel-oped process; however, certain parameters are suspected of controlling separation, including buffer concentration, half cycle time, packed column height, and reservoir displacement volume. Results show that ratios of bottom product concentration to top product concentration can be sustained at approximately 2.0, using the semi-continuous mode.



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