Document Type


Date of Award


Degree Name

Master of Science in Chemical Engineering - (M.S.)


Chemical Engineering and Chemistry

First Advisor

Hung T. Chen

Second Advisor

George Y. Lei

Third Advisor

Gordon Lewandowski


Continuous pH-parametric pumping separations of a haemoglobin - albumin - CM Separose system were experimentally investigated. The parapump system has a feed, containing the protein mixture to be separated, introduced alternately to the top and bottom of the chromatographic column. The top and bottom products are withdrawn from the apparatus during the bottom and top feed respectively. It is shown that under certain conditions the pH-driven parametric pump has the capacity for removal of a protein component from one product fraction and large enrichment in the other fraction. Moreover, the continuous process can be operated with a large feed throughput.



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