Document Type
Thesis
Date of Award
6-30-1979
Degree Name
Master of Science in Chemical Engineering - (M.S.)
Department
Chemical Engineering and Chemistry
First Advisor
Hung T. Chen
Second Advisor
George Y. Lei
Third Advisor
Gordon Lewandowski
Abstract
Continuous pH-parametric pumping separations of a haemoglobin - albumin - CM Separose system were experimentally investigated. The parapump system has a feed, containing the protein mixture to be separated, introduced alternately to the top and bottom of the chromatographic column. The top and bottom products are withdrawn from the apparatus during the bottom and top feed respectively. It is shown that under certain conditions the pH-driven parametric pump has the capacity for removal of a protein component from one product fraction and large enrichment in the other fraction. Moreover, the continuous process can be operated with a large feed throughput.
Recommended Citation
Wong, Yuk-Wei, "Continuous fractionation of protein mixtures by pH-parametric pumping" (1979). Theses. 2075.
https://digitalcommons.njit.edu/theses/2075
