Parametric pumping with pH and ionic strength: Enzyme purification

Document Type

Article

Publication Date

1-1-1981

Abstract

Purification of alkaline phosphatase was experimentally investigated by semicontinuous parametric pumping. The parapumping involves reciprocating flow of the enzyme mixture to be purified through a bed of ion exchanger and, simultaneously, synchronous cyclic variation of the pH and ionic strength. The change of pH and ionic strength displaces the interphase equilibrium and, in combination with the reciprocating flow, causes preferential movement of the sorbable components of the mixture toward one end of the bed, leading to a buildup of the separation from cycle to cycle. It is shown that the parametric pumping can yield a larger enzyme activity recovered with high purification factors than the conventional cycling-zone adsorption process. © 1981 American Chemical Society.

Identifier

0019565799 (Scopus)

Publication Title

Industrial and Engineering Chemistry Fundamentals

External Full Text Location

https://doi.org/10.1021/i100002a012

ISSN

01964313

First Page

171

Last Page

174

Issue

2

Volume

20

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