In vitro investigation of the procoagulant properties of silver ion

Document Type

Conference Proceeding

Publication Date

1-1-2004

Abstract

Silver ion, a potent antimicrobial and procoagulant, is hypothesized to achieve its haemostatic effect by binding anticoagulant proteins. We performed in vitro studies to gain more insight into its mechanism of action. 10 μL of 5% AgNO3 was added to 1 mL human plasma (n=4), forming a precipitate. The precipitate was recovered through centrifugation and iteratively washed with saline. The supernatant(s) and precipitate were mixed with Coomassie blue stain and protein content was measured via spectrophotometric absorbance. An analysis of variance indicated significant (p<0.0001) differences between all groups. Multiple comparison tests showed a significant (p<0.001) increase in absorbance from the final wash and dye blank to the precipitate, indicating the presence of proteins in the precipitate. Silver ion may be precipitating anticoagulant proteins as its mechanism of action.

Identifier

2942596087 (Scopus)

Publication Title

Proceedings of the IEEE Annual Northeast Bioengineering Conference Nebec

e-ISSN

21607001

ISSN

1071121X

First Page

178

Last Page

179

Volume

30

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