Document Type

Thesis

Date of Award

Fall 12-31-2017

Degree Name

Master of Science in Biopharmaceutical Engineering - (M.S.)

Department

Chemical, Biological and Pharmaceutical Engineering

First Advisor

Piero M. Armenante

Second Advisor

S. Basuray

Third Advisor

Roman Wolodymyr Voronka

Abstract

In microfluidics, efficiency and mixing time are the greatest disadvantages. These parameters hinder the application of microfluidic devices for biochemical and immunological assays. However, once these disadvantages have been overcome by optimizing the parameters of the microfluidic device, it becomes the important analytical tool. In this experiment, various designs of microfluidic devices have been both simulated using COMSOL software, and experimentally verified to obtain the optimized parameter such as depth and velocity for better mixing efficiency. The COMSOL model has been validated by comparing the results with fluorescent images data of the experiment. The microfluidic device is built with Adhesive double-sided tape and glass slides. The microfluidic channels are 25µm in depth and 700µm in width. These channels have a serpentine design with three loops. It was been analyzed that by increasing the depth of the channel to 400µm at 0.1µl/min flow rate, 99.5% mixing efficiency was obtained. As both the contact area and time were increasing, which in turn lead to the increase in diffusion mixing between the two streams.

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