Document Type


Date of Award

Fall 1-31-2016

Degree Name

Master of Science in Chemistry - (M.S.)


Chemistry and Environmental Science

First Advisor

Edgardo Tabion Farinas

Second Advisor

Haidong Huang

Third Advisor

Zafar Iqbal


Oxidations of DNA nucleobases are believed to be one of the sources of aging and some age-dependent disease, such as cancer. Human body have various mechanisms to repair damaged DNA and base-excision-repair is one mechanism for removing those oxidized nucleobases. This mechanism processes in the cells by cutting the oxidized nucleobases from deoxyribose and letting them excrete though urine. Detection of those abnormal nucleobases in urine has been suggested as a convenient way of early cancer diagnosis. 5-OHU (5-hydroxyuracil), the oxidation product of cytosine, is one of those abnormal nucleobases and it potentially forms base pairs with all four DNA nucleobases, which may in the end cause mutation. In this project, a binding cavity for 5-OHU in triplex system was designed and triplex conjugated gold nanoparticles were synthesized. The melting temperature of the triplex DNA-GNP conjugates were 5-OHU concentration dependent, which demonstrated the potential of the system for colorimetric detection of urinary 5-OHU.

Included in

Chemistry Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.