Date of Award
Master of Science in Chemistry - (M.S.)
Chemistry and Environmental Science
Edgardo Tabion Farinas
Oxidations of DNA nucleobases are believed to be one of the sources of aging and some age-dependent disease, such as cancer. Human body have various mechanisms to repair damaged DNA and base-excision-repair is one mechanism for removing those oxidized nucleobases. This mechanism processes in the cells by cutting the oxidized nucleobases from deoxyribose and letting them excrete though urine. Detection of those abnormal nucleobases in urine has been suggested as a convenient way of early cancer diagnosis. 5-OHU (5-hydroxyuracil), the oxidation product of cytosine, is one of those abnormal nucleobases and it potentially forms base pairs with all four DNA nucleobases, which may in the end cause mutation. In this project, a binding cavity for 5-OHU in triplex system was designed and triplex conjugated gold nanoparticles were synthesized. The melting temperature of the triplex DNA-GNP conjugates were 5-OHU concentration dependent, which demonstrated the potential of the system for colorimetric detection of urinary 5-OHU.
Xing, Qingyu, "Triplex dna receptor-gold nanoparticle conjugates for detection of oxidized dna base" (2015). Theses. 265.