Document Type


Date of Award


Degree Name

Master of Science in Chemical Engineering - (M.S.)


Chemical Engineering and Chemistry

First Advisor

Hung T. Chen

Second Advisor

Teddy Greenstein

Third Advisor

Jerome J. Salamone


Parametric pumping represents a new development in separation science. It has attracted considerable attention both because of its novelty and because it permits continuous operation in small equipment with very high separation factors. The basic principle of parametric pumping is to utilize the coupling of periodic changes in equilibrium conditions caused by periodic changes in some intensive variables (temperature, pH, electric field, etc.), and periodic changes in flow direction to separate the components of fluid which flows past a solid adsorbent. Applications of parametric pumping involving the separation of valuable materials such as proteins would be very attractive and profitable to investigate.

Many proteins are often processed batchwise. Parametric pumping offers the possibility of continuous processing, thereby tending to minimize both processing time and degradation. The overall objective of this research is to determine the feasibility of operating a semi-continuous pH-parametric pump for protein separation. The model system used is hemoglobin-albumin on sephadex ion exchange. It is hoped that the results of this work would be general enough to be invaluable in the separation of binary or multi-protein mixtures, and will provide necessary technical information for the design of full-scale parametric pumps with a sound engineering and economic basis.



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