Date of Award
Master of Science in Chemical Engineering - (M.S.)
Chemical Engineering and Chemistry
Hung T. Chen
Mahmoud F. Abd-El-Bary
Voidage of SP Sephadex (C-50) ion exchanger at different aqueous buffer concentration and the pH have been estimated from draining the interstitial aqueous. Experimental results of the SP Sephadex (C-50) voidage at pH cycling zone are in quantitative agreement with previous test method, so the voidage estimated from draining the interstital aqueous can be used in pH-parametric pumping. The optional displacement volume in semicontinuous pH-parametric pump of different buffer concentration can be predicted from voidage. A semicontinuous pH-parametric pump for separating proteins has been experimentally investigated using the model system haemoglobin and albumin on a. SP Sephadex (C-50) ion exchanger. The pump considered has a center feed between an enriching column and a stripping column, and is operated batchwise during upflow and continuously during downflow. It has been shown that parametric pump is capable of separating proteins with high separation factor. Displacement volume and the product flow rate of the product stream have been found to be important in determining the pumping performance. The cycle time and the location of the feed introduced affect separation in some condition. Separation can be improved with large displacement volume under the optional displacement volume. The components removed can be concentrated to any desired practical level by setting the flow rate of the product stream containing these components at the required value.
Lee, Hsien-Chih, "Semicontinous pH-parametric pump : experimental study" (1978). Theses. 2121.