Date of Award
Master of Science in Chemical Engineering - (M.S.)
Chemical Engineering and Chemistry
Hung T. Chen
Ernest N. Bart
The technique of parametric pumping represents a new and valuable development in the separation science of proteins. Many conventional protein separation are often processed batchwise. However, parametric pumping offers the possibility of continuous processing; the continuous process provides both the advantages of decreasing the processing time and of minimizing degradation.
Preliminary step testing experiments were conducted to study the equilibrium of protein between the stationary and mobile phase of the sephadex ion exchanger.
It was the purpose of this research to study the feasibility of operating a semi-continuous parametric pump with a sephadex ion exchanger for the separation of a manmade protein mixture of albumin and hemaglobin. Factors affecting separation performance, such as concentration and pH were investigated experimentally.
It was found that an increase in the buffer concentration and, hence, in the sodium counter ion concentration results in a shifting of position of equilibrium involving the ion exchanger. This results in less uptake of hemoglobin and high concentration in the top product stream. The albumin concentration is unaffected by the pumping operation. In both top and bottom product streams, it remains essentially at the feed concentration.
It also been concluded that change of pH towards the isoelectric point of the protein renders it neutral and thus reduces interaction with the ion exchanger, less separation is found at a smaller spread of pH values. A larger spread of pH values will cause significant increase in separation.
Hsieh, Tsu-kun S., "Effect of concentration and pH on parametric pumping separations" (1978). Theses. 2100.