Date of Award

Spring 1979

Document Type

Thesis

Degree Name

Master of Science in Chemical Engineering - (M.S.)

Department

Chemical Engineering and Chemistry

First Advisor

Teddy Greenstein

Second Advisor

Deran Hanesian

Third Advisor

John E. McCormick

Abstract

A continuous fermentation assembly was constructed and studies on the growth of the organism, Vibrio succinogenes, and its production of asparaginase was conducted. Results were compared with that already performed on a batch system and showed that a continuous fermentation produced 31.83 I.U. enzymes/mM ammonium formate compared to 17.4 I.U. enzyme/mM formate for batch. Some of the runs performed indicated that the organism was washing out or giving very low specific growth rates. It was decided that this was not due to operational parameters of the chemostat, but possible chemical contamination in the medium. With all things considered, a continuous fermentation on producing the enzyme, asparaginase, was almost doubled when compared with that of batch making this form of fermentation a viable alternative.

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