Document Type


Date of Award

Spring 5-31-1989

Degree Name

Master of Science in Environmental Science - (M.S.)


Chemical Engineering, Chemistry and Environmental Science

First Advisor

Samir S. Sofer

Second Advisor

Richard B. Trattner

Third Advisor

Peter F. Strom


Activated sludges acclimated with phenol and 2-chlorophenol in one case, and a mixture of benzene, toluene, and o, in and p-xylene in another case, were characterized for biodegradation of 2-chlorophenol and benzene, respectively. Activated sludge was obtained from the Parsippany-Troy Hills Water Pollution Control Plant (NJ).

For both cases colonies were isolated before acclimation, after acclimation, and at the end of biodegradation studies. Since the unacclimated group was the same in both cases, there were a total of five different groups for which characterization was carried out.

For all the five groups together, a total of 54 isolates were obtained and 35 of these could be assigned to a genus. The genera assigned included Bacillus, Lactobacillus, Microbacterium, Plesiomonas, Kurthia, Kiebsiella, Corynebacterium and Pseudomonas.

Studies done with individual end run isolates and their mixture, in shaker flasks, established that the primary degrader of 2-chlorophenol was a Bacillus and that of benzene was a Pseudomonas.