Date of Award

Fall 1994

Document Type

Thesis

Degree Name

Master of Science in Environmental Science - (M.S.)

Department

Chemical Engineering, Chemistry and Environmental Science

First Advisor

Piero M. Armenante

Second Advisor

David Kafkewitz

Third Advisor

Richard B. Trattner

Abstract

Recent investigations of volcanic smoker chimneys along the Atlantic Rift has lead to the discovery of hyperthermophilic bacteria. In particular, Pyrococcus GBD and Desulfurococcus SY were studied, optimizing growth conditions. This was achieved using elemental sulfur amendments, artificial sea water, and dehydrated marine broth. The microbes were then acclimated to enriched cultures containing 2,4,6 trichlorophenol in 0.0126 mM, 0.063 mM, and 0.126 mM amendments. The amended cultures showed dehalogenating properties and were subjected to a data qualifying program. This program indicated that the dehalogenation was a function of microbial gowth. A mass balance experiment showed that both microbes were able to reductively dehalogenate 0.5 mM 2,4,6 TCP to 2,6 DCP, 2 MCP, and finally phenol in a quantitative manner.

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