Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein
Document Type
Article
Publication Date
4-11-2017
Abstract
Two cationic conjugated polyelectrolytes (CPEs) based on poly(phenylene ethynylene) are found to exhibit efficient 2-photon excited fluorescence. In particular, the CPE aggregates display enhanced 2-photon absorption compared to the nonaggregated states. The CPEs are used as 2-photon absorption sensitizers to harvest light and amplify the fluorescence from green fluorescent protein (GFP) through Förster resonance energy transfer. Considerably enhanced GFP fluorescence under near-infrared 2-photon excitation is demonstrated both in solution and in HeLa cells. The results suggest applications in 2-photon fluorescence imaging of cells, tissues, and organs of living animals expressing GFP.
Identifier
85017408261 (Scopus)
Publication Title
Chemistry of Materials
External Full Text Location
https://doi.org/10.1021/acs.chemmater.7b00676
e-ISSN
15205002
ISSN
08974756
First Page
3295
Last Page
3303
Issue
7
Volume
29
Grant
HDTRA1-08-1-0053
Fund Ref
Defense Threat Reduction Agency
Recommended Citation
Wang, Shanshan; Li, Zhiliang; Liu, Xinglei; Phan, Samantha; Lv, Fengting; Belfield, Kevin D.; Wang, Shu; and Schanze, Kirk S., "Two-Photon Absorption of Cationic Conjugated Polyelectrolytes: Effects of Aggregation and Application to 2-Photon-Sensitized Fluorescence from Green Fluorescent Protein" (2017). Faculty Publications. 9630.
https://digitalcommons.njit.edu/fac_pubs/9630
