Hyaluronic acid-laminin hydrogels increase neural stem cell transplant retention and migratory response to SDF-1α

Document Type

Article

Publication Date

7-1-2017

Abstract

The chemokine SDF-1α plays a critical role in mediating stem cell response to injury and disease and has specifically been shown to mobilize neural progenitor/stem cells (NPSCs) towards sites of neural injury. Current neural transplant paradigms within the brain suffer from low rates of retention and engraftment after injury. Therefore, increasing transplant sensitivity to injury-induced SDF-1α represents a method for increasing neural transplant efficacy. Previously, we have reported on a hyaluronic acid-laminin based hydrogel (HA-Lm gel) that increases NPSC expression of SDF-1α receptor, CXCR4, and subsequently, NPSC chemotactic migration towards a source of SDF-1α in vitro. The study presented here investigates the capacity of the HA-Lm gel to promote NPSC response to exogenous SDF-1α in vivo. We observed the HA-Lm gel to significantly increase NPSC transplant retention and migration in response to SDF-1α in a manner critically dependent on signaling via the SDF-1α-CXCR4 axis. This work lays the foundation for development of a more effective cell therapy for neural injury, but also has broader implications in the fields of tissue engineering and regenerative medicine given the essential roles of SDF-1α across injury and disease states.

Identifier

85009854473 (Scopus)

Publication Title

Matrix Biology

External Full Text Location

https://doi.org/10.1016/j.matbio.2016.09.007

e-ISSN

15691802

ISSN

0945053X

PubMed ID

27645115

First Page

206

Last Page

216

Volume

60-61

Grant

DP2HD084067

Fund Ref

National Institutes of Health

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