Quantifying the membrane potential during E. coli growth stages

Document Type

Article

Publication Date

2-1-2010

Abstract

The presence of the resting membrane potential has a strong effect on the dielectric behavior of cell suspensions. Using this observation and a well-established theoretical model, the low frequency dielectric dispersion curves of E. coli cell suspensions are de-convoluted to obtain the resting membrane potential of E. coli cells at various growth stages. Four regions of the exponential growth stage are investigated and the measurements indicate that the membrane depolarizes from - 220 mV in the early exponential phase to - 140 mV in the late exponential phase. The conductivity of the cell suspension is also found to decrease as the cells progress from the early to the late exponential phases. © 2009 Elsevier B.V. All rights reserved.

Identifier

72749109322 (Scopus)

Publication Title

Biophysical Chemistry

External Full Text Location

https://doi.org/10.1016/j.bpc.2009.11.005

ISSN

03014622

PubMed ID

20031298

First Page

133

Last Page

137

Issue

2-3

Volume

146

This document is currently not available here.

Share

COinS