Luminescent probes for ultrasensitive detection of nucleic acids

Document Type

Article

Publication Date

2-17-2010

Abstract

Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of cDNA oligonucleotides by molecular beacons. Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. This value is about 50 to 100 times more sensitive than the level achieved with conventional fluorescencebased molecular beacons, and is 10 to 60 times more sensitive than previously reported for other lanthanidebased hybridization probes. These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications. © 2010 American Chemical Society.

Identifier

77049105211 (Scopus)

Publication Title

Bioconjugate Chemistry

External Full Text Location

https://doi.org/10.1021/bc900403n

e-ISSN

15204812

ISSN

10431802

PubMed ID

20085336

First Page

319

Last Page

327

Issue

2

Volume

21

Grant

R01MH079197

Fund Ref

National Institute of Mental Health

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