Development of Schwann cell-seeded conduit using chitosan-based biopolymers for nerve repair

Document Type

Conference Proceeding

Publication Date

6-8-2010

Abstract

The purpose of this study is to develop biodegradable nerve guidance conduits seeded with Schwann cells (SC) using chitosan-based biopolymers for improving nerve regeneration. Chitosan, prepared from alkaline hydrolysis of chitin, is a natural polysaccharide biopolymer, having biocompatibility and biodegradability. In this study, chitosan and chitosan-gelatin membranes were prepared, characterized, and evaluated. The SC isolated from rat sciatic nerve was seeded on chitosan and chitosan-gelatin membranes to investigate their interactions. To enhance the SC attachment and growth on the membranes, we incorporated the poly-L-lysine (PLL) into chitosan and chitosan-gelatin network at various ratios of 1:800, 1:200, 1:50 (chitosan or chitosan-gelatin: PLL). We have also investigated the effect of extracellular matrix (ECM) molecules on the proliferation of SC. We observed that SC proliferation on PLL-coated surface was significantly higher than that of ECM coated and uncoated surfaces. With the increase of PLL ratio into chitosan or chitosan-gelatin network, the attachment and growth of SCs were significantly enhanced. This study indicates that the incorporation of PLL into chitosan and chitosan-gelatin membranes significantly improves the bioactivity of the materials. The Schwann cell-seeded films can be used to construct a three-dimensional nerve conduit for enhancing nerve regeneration. ©2010 IEEE.

Identifier

77953062033 (Scopus)

ISBN

[9781424468799]

Publication Title

Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference Nebec 2010

External Full Text Location

https://doi.org/10.1109/NEBC.2010.5458176

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