N-type Ca2+ channels carry the largest current: Implications for nanodomains and transmitter release

Authors

Alexander M. Weber, Krembil Research Institute
Fiona K. Wong, Krembil Research Institute
Adele R. Tufford, Krembil Research Institute
Lyanne C. Schlichter, Krembil Research Institute
Victor Matveev, New Jersey Institute of Technology
Elise F. Stanley, Krembil Research Institute

Document Type

Article

Publication Date

11-1-2010

Abstract

Presynaptic terminals favor intermediate-conductance Ca V2.2 (N type) over high-conductance Ca V 1 (L type) channels for single-channel, Ca²⁺ nanodomain-triggered synaptic vesicle fusion. However, the standard Ca V 1>Ca V 2>Ca V 3 conductance hierarchy is based on recordings using nonphysiological divalent ion concentrations. We found that, with physiological Ca²⁺ gradients, the hierarchy was Ca V 2.2>Ca V 1>Ca V 3. Mathematical modeling predicts that the Ca V 2.2 Ca²⁺ nanodomain, which is ĝ̂1/425% more extensive than that generated by Ca V 1, can activate a calcium-fusion sensor located on the proximal face of the synaptic vesicle. © 2010 Nature America, Inc. All rights reserved.

Identifier

78049310920 (Scopus)

Publication Title

Nature Neuroscience

External Full Text Location

https://doi.org/10.1038/nn.2657

ISSN

10976256

PubMed ID

20953196

First Page

1348

Last Page

1350

Issue

11

Volume

13

Grant

MT-13657

Fund Ref

National Science Foundation

Recommended Citation

Weber, Alexander M.; Wong, Fiona K.; Tufford, Adele R.; Schlichter, Lyanne C.; Matveev, Victor; and Stanley, Elise F., "N-type Ca2+ channels carry the largest current: Implications for nanodomains and transmitter release" (2010). Faculty Publications. 6030.
https://digitalcommons.njit.edu/fac_pubs/6030