"Microtoxicology by microfluidic instrumentation: a review" by Jialan Cao, Charmi Chande et al.
 

Microtoxicology by microfluidic instrumentation: a review

Document Type

Article

Publication Date

6-1-2022

Abstract

Microtoxicology is concerned with the toxic effects of small amounts of substances. This review paper discusses the application of small amounts of noxious substances for toxicological investigation in small volumes. The vigorous development of miniaturized methods in microfluidics over the last two decades involves chip-based devices, micro droplet-based procedures, and the use of micro-segmented flow for microtoxicological studies. The studies have shown that the microfluidic approach is particularly valuable for highly parallelized and combinatorial dose-response screenings. Accurate dosing and mixing of effector substances in large numbers of microcompartments supplies detailed data of dose-response functions by highly concentration-resolved assays and allows evaluation of stochastic responses in case of small separated cell ensembles and single cell experiments. The investigations demonstrate that very different biological targets can be studied using miniaturized approaches, among them bacteria, eukaryotic microorganisms, cell cultures from tissues of multicellular organisms, stem cells, and early embryonic states. Cultivation and effector exposure tests can be performed in small volumes over weeks and months, confirming that the microfluicial strategy is also applicable for slow-growing organisms. Here, the state of the art of miniaturized toxicology, particularly for studying antibiotic susceptibility, drug toxicity testing in the miniaturized system like organ-on-chip, environmental toxicology, and the characterization of combinatorial effects by two and multi-dimensional screenings, is discussed. Additionally, this review points out the practical limitations of the microtoxicology platform and discusses perspectives on future opportunities and challenges.

Identifier

85131783791 (Scopus)

Publication Title

Lab on A Chip

External Full Text Location

https://doi.org/10.1039/d2lc00268j

e-ISSN

14730189

ISSN

14730197

PubMed ID

35678285

First Page

2600

Last Page

2623

Issue

14

Volume

22

Grant

2016FE9016

Fund Ref

Bundesministerium für Bildung und Forschung

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