"Effect of crosslinking, hydroxyapatite addition, and fiber alignment t" by Felipe Castro Menezes, Nataly Machado Siqueira et al.
 

Effect of crosslinking, hydroxyapatite addition, and fiber alignment to stimulate human mesenchymal stem cells osteoinduction in polycaprolactone-based electrospun scaffolds

Document Type

Article

Publication Date

9-1-2022

Abstract

Electrospinning is a versatile technique for producing composite scaffolds with nanostructure properties similar to the natural extracellular matrix. Biomaterials possessing mechanical, structural, and biological properties required for bone tissue engineering are a big challenge. However, the effect of fiber alignment, their mechanical properties, and chemical modifications on fibers are usually investigated individually. In this study, PCL/GE/HA scaffolds were electrospun in a static and drum rotatory collector to investigate the effects of alignment on the physicochemical properties of composite scaffolds. Furthermore, to achieve a stable composite with natural polymer gelatin (GE), a water-soluble, zero-length crosslinker (N-[3-dimethylaminopropyl]-N′-ethylcarbodiimide hydrochloride, EDC) was used to crosslink GE. Our results have outlined that the incorporation of GE, as well as crosslinking process, produced a hydrophilic biomaterial, improving wettability compared to pure polycaprolactone (PCL). In addition, the alignment reinforced the material, increasing mechanical strength. Biological tests showed that GE addition and the alignment allowed a better osteoinduction than pure random PCL.

Identifier

85129633582 (Scopus)

Publication Title

Polymers for Advanced Technologies

External Full Text Location

https://doi.org/10.1002/pat.5723

e-ISSN

10991581

ISSN

10427147

First Page

2682

Last Page

2695

Issue

9

Volume

33

Fund Ref

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

This document is currently not available here.

Share

COinS