Semicontinuous Protein Separation via Variable-Field-Strength Electrophoresis

Document Type

Article

Publication Date

1-1-1985

Abstract

Variable-field-strength electrophoresis is proposed as a new semicontinuous separation process for protein mixtures. Conventional preparative-scale polyacrylamide gel electrophoresis equipment is used, but new feed is added in successive cycles of operation and the gel Is reused for a number of cycles rather than dismantling the system and polymerizing new gel in situ for each feed sample. Experimental results for the new process are presented, based on the separation of a mixture of human hemoglobin and human serum albumin. Two modes of operation are investigated with the electric field being switched from positive to negative polarity or from low to high voltage during each cycle. © 1985, American Chemical Society. All rights reserved.

Identifier

0022162571 (Scopus)

Publication Title

Industrial and Engineering Chemistry Fundamentals

External Full Text Location

https://doi.org/10.1021/i100020a014

ISSN

01964313

First Page

489

Last Page

497

Issue

4

Volume

24

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