A comparative study of biphasic calcium phosphate ceramics for human mesenchymal stem-cell-induced bone formation

Document Type

Article

Publication Date

6-1-2005

Abstract

For the repair of bone defects, a tissue engineering approach would be to combine cells capable of osteogenic (i.e. bone-forming) activity with an appropriate scaffolding material to stimulate bone regeneration and repair. Human mesenchymal stem cells (hMSCs), when combined with hydroxyapatite/β- tricalcium phosphate (HA/TCP) ceramic scaffolds of the composition 60% HA/40% TCP (in weight %), have been shown to induce bone formation in large, long bone defects. However, full repair or function of the long bone could be limited due to the poor remodeling of the HA/TCP material. We conducted a study designed to determine the optimum ratio of HA to TCP that promoted hMSC induced bone formation yet be fully degradable. In a mouse ectopic model, by altering the composition of HA/TCP to 20% HA/80% TCP, hMSC bone induction occurred at the fastest rate in vivo over the other formulations of the more stable 100% HA, HA/TCP (76/24, 63/37, 56/44), and the fully degradable, 100% TCP. In vitro studies also demonstrated that 20/80 HA/TCP stimulated the osteogenic differentiation of hMSCs as determined by the expression of osteocalcin. © 2004 Elsevier Ltd. All rights reserved.

Identifier

11144350098 (Scopus)

Publication Title

Biomaterials

External Full Text Location

https://doi.org/10.1016/j.biomaterials.2004.09.035

ISSN

01429612

PubMed ID

15621253

First Page

3631

Last Page

3638

Issue

17

Volume

26

Grant

BES-02747

This document is currently not available here.

Share

COinS