Temporal analysis of vascular smooth muscle cell elasticity and adhesion reveals oscillation waveforms that differ with aging
Document Type
Article
Publication Date
10-1-2012
Abstract
A spectral analysis approach was developed for detailed study of time-resolved, dynamic changes in vascular smooth muscle cell (VSMC) elasticity and adhesion to identify differences in VSMC from young and aged monkeys. Atomic force microscopy (AFM) was used to measure Young's modulus of elasticity and adhesion as assessed by fibronectin (FN) or anti-beta 1 integrin interaction with the VSMC surface. Measurements demonstrated that VSMC cells from old vs. young monkeys had increased elasticity (21.6kPa vs. 3.5kPa or a 612% increase in elastic modulus) and adhesion (86pN vs. 43pN or a 200% increase in unbinding force). Spectral analysis identified three major frequency components in the temporal oscillation patterns for elasticity (ranging from 1.7×10-3 to 1.9×10-2Hz in old and 8.4×10-4 to 1.5×10-2 Hz in young) and showed that the amplitude of oscillation was larger (P<0.05) in old than in young at all frequencies. It was also observed that patterns of oscillation in the adhesion data were similar to the elasticity waveforms. Cell stiffness was reduced and the oscillations were inhibited by treatment with cytochalasin D, ML7 or blebbistatin indicating the involvement of actin-myosin-driven processes. In conclusion, these data demonstrate the efficacy of time-resolved analysis of AFM cell elasticity and adhesion measurements and that it provides a uniquely sensitive method to detect real-time functional differences in biomechanical and adhesive properties of cells. The oscillatory behavior suggests that mechanisms governing elasticity and adhesion are coupled and affected differentially during aging, which may link these events to changes in vascular stiffness. © 2012 The Authors. Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland.
Identifier
84866459956 (Scopus)
Publication Title
Aging Cell
External Full Text Location
https://doi.org/10.1111/j.1474-9726.2012.00840.x
e-ISSN
14749726
ISSN
14749718
PubMed ID
22639979
First Page
741
Last Page
750
Issue
5
Volume
11
Grant
R01HL062863
Fund Ref
National Heart, Lung, and Blood Institute
Recommended Citation
Zhu, Yi; Qiu, Hongyu; Trzeciakowski, Jerome P.; Sun, Zhe; Li, Zhaohui; Hong, Zhongkui; Hill, Michael A.; Hunter, William C.; Vatner, Dorothy E.; Vatner, Stephen F.; and Meininger, Gerald A., "Temporal analysis of vascular smooth muscle cell elasticity and adhesion reveals oscillation waveforms that differ with aging" (2012). Faculty Publications. 18088.
https://digitalcommons.njit.edu/fac_pubs/18088
