The influence of piezoelectric scaffolds on neural differentiation of human neural stem/progenitor cells

Document Type

Article

Publication Date

10-1-2012

Abstract

Human neural stem/progenitor cells (hNSCs/NPCs) are a promising cell source for neural tissue engineering because of their ability to differentiate into various neural lineages. In this study, hNSC/NPC differentiation was evaluated on piezoelectric, fibrous scaffolds. These smart materials have an intrinsic material property where transient electric potential can be generated in the material upon minute mechanical deformation. hNSCs/NPCs cultured on the scaffolds and films differentiated into β-III tubulin-positive cells, a neuronal cell marker, with or without the presence of inductive factors. In contrast, hNSCs/NPCs cultured on laminin-coated plates were predominantly nestin positive, a NSC marker, in the control medium. Gene expression results suggest that the scaffolds may have promoted the formation of mature neural cells exhibiting neuron-like characteristics. hNSCs/NPCs differentiated mostly into β-III tubulin-positive cells and had the greatest average neurite length on micron-sized, annealed (more piezoelectric), aligned scaffolds, demonstrating their potential for neural tissue-engineering applications. © Copyright 2012, Mary Ann Liebert, Inc.

Identifier

84867027838 (Scopus)

Publication Title

Tissue Engineering Part A

External Full Text Location

https://doi.org/10.1089/ten.tea.2011.0540

e-ISSN

1937335X

ISSN

19373341

PubMed ID

22646285

First Page

2063

Last Page

2072

Issue

19-20

Volume

18

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