Protein adsorption dissociation constants in various types of biochromatography

Document Type

Article

Publication Date

1-1-1999

Abstract

The equilibrium kinetics, adsorption and desorption of various proteins on Chromatographie adsorbents have been widely studied and published in the literature. What is lacking is a comprehensive review of the available information. In this article the equilibrium data has been summarized and presented in the form of dissociation constants. This summary leads to a chart of relative protein adsorption strength of various types of chromatography in terms of dissociation constants. Some of the factors affecting dissociation constants such as ligand concentration, pH, ionic strength and temperature have also been discussed. The results of this study can be used as important information for design, development, scale up and optimization of Chromatographic processes for protein separations. © 1998 Elsevier Science B. V. All rights reserved.

Identifier

33751040389 (Scopus)

Publication Title

Studies in Surface Science and Catalysis

External Full Text Location

https://doi.org/10.1016/s0167-2991(99)80573-4

ISSN

01672991

First Page

829

Last Page

845

Volume

120 A

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