High-Throughput Generation, Manipulation, and Degradation of Magnetic Nanoparticle-Laden Alginate Core-Shell Beads for Single Bacteria Culturing Analysis
Document Type
Article
Publication Date
7-1-2023
Abstract
Microbes could be found almost everywhere around us and have significant impacts on our human society. The treatment of microorganisms has long been seen as a complex problem. Till now, most of the genetic and phenotypic information regarding rare species is buried in the bulk microbial colony due to a lack of efficient tools to screen live bacteria. Droplet microfluidics offers a powerful approach to address this problem. However, the interactions among bacteria and their living environment are entirely restricted by the water/oil interfaces in conventional water/oil single emulsion-based microfluidic systems. Here, we demonstrate an oil-mediated all-aqueous microfluidic workflow that can overcome this drawback. In contrast to the previous works, our all-aqueous culturing environment allows cell-cell and cell-environment interactions, thus facilitating the growth of bacteria. Fe3O4 magnetic nanoparticles added into the alginate beads enables on-chip manipulation of the microcapsules. The core-shell structure separately encapsulates bacteria and magnetic particles in the core and shell to avoid contamination. We demonstrate the feasibility of this approach by single bacterium culturing in droplet-templated alginate beads. Finally, a new approach is proposed to degrade the alginate beads for post-treatment. This novel microfluidic workflow can create new opportunities for microbial applications, such as bacteria culturing and screening.
Identifier
85137878551 (Scopus)
Publication Title
IEEE Transactions on Nanobioscience
External Full Text Location
https://doi.org/10.1109/TNB.2022.3205057
e-ISSN
15582639
ISSN
15361241
PubMed ID
36074887
First Page
487
Last Page
497
Issue
3
Volume
22
Grant
21922816
Fund Ref
National Natural Science Foundation of China
Recommended Citation
Yuan, Shuai; Zhang, Yulin; Nan, Lang; Lai, P. T.; Zhang, Tong; Pong, Philip W.T.; and Shum, Ho Cheung, "High-Throughput Generation, Manipulation, and Degradation of Magnetic Nanoparticle-Laden Alginate Core-Shell Beads for Single Bacteria Culturing Analysis" (2023). Faculty Publications. 1607.
https://digitalcommons.njit.edu/fac_pubs/1607
