"Elastomeric platform with surface wrinkling patterns to control cardia" by Andrew House, Jason Cornick et al.
 

Elastomeric platform with surface wrinkling patterns to control cardiac cell alignment

Document Type

Article

Publication Date

8-1-2023

Abstract

There is a growing interest in creating 2D cardiac tissue models that display native extracellular matrix (ECM) cues of the heart tissue. Cellular alignment alone is known to be a crucial cue for cardiac tissue development by regulating cell–cell and cell-ECM interactions. In this study, we report a simple and robust approach to create lamellar surface wrinkling patterns enabling spatial control of pattern dimensions with a wide range of pattern amplitude (A ≈ 2–55 μm) and wavelength (λ ≈ 35–100 μm). For human cardiomyocytes (hCMs) and human cardiac fibroblasts (hCFs), our results indicate that the degree of cellular alignment and pattern recognition are correlated with pattern A and λ. We also demonstrate fabrication of devices composed of micro-well arrays with user-defined lamellar patterns on the bottom surface of each well for high-throughput screening studies. Results from a screening study indicate that cellular alignment is strongly diminished with increasing seeding density. In another study, we show our ability to vary hCM/hCF seeding ratio for each well to create co-culture systems where seeding ratio is independent of cellular alignment.

Identifier

85147579313 (Scopus)

Publication Title

Journal of Biomedical Materials Research Part A

External Full Text Location

https://doi.org/10.1002/jbm.a.37511

e-ISSN

15524965

ISSN

15493296

PubMed ID

36762538

First Page

1228

Last Page

1242

Issue

8

Volume

111

Fund Ref

New Jersey Health Foundation

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