Gene expression profiling of acute spinal cord injury reveals spreading inflammatory signals and neuron loss

Document Type

Article

Publication Date

1-1-2002

Abstract

We have completed the first large-scale gene expression study of acute spinal cord injury (SCI) in rat. Oligonucleotide microarrays containing 1,200 gene-specific probes were used to quantify mRNA levels, relative to uninjured controls, in spinal cords injured using a standard contusion model. Our results revealed a marked loss of neuron-specific mRNAs at the injury site. The surviving cells showed a characteristic inflammatory response that started at the injury site and spread to the distal cord. Changes in several mRNA levels were associated with putative regenerative responses in the spinal cord. Notably, phosphodiesterase 4, nestin, glia-derived neurite promoting factor, and GAP-43 mRNAs increased significantly. Other mRNAs clustered temporally and spatially with these regeneration-associated genes. Thus we have described global patterns of gene expression following acute SCI, and we have identified targets for future study and possible therapeutic intervention. spinal cord injury; microarray; gene expression; regeneration-associated genes; inflammation; cell death.

Identifier

0348000947 (Scopus)

Publication Title

Physiological Genomics

ISSN

15312267

PubMed ID

11773606

First Page

201

Last Page

213

Issue

7

Volume

2002

This document is currently not available here.

Share

COinS