Comparison of various measures of microbial growth kinetics in suspended and biofilm cultures during biodegradation of naphthalene

Document Type

Article

Publication Date

1-1-2002

Abstract

This research focused on measurement of bacterial growth rates and activities in suspended cultures and biofilms using Pseudomonas putida (ATCC 17484) for biodegradation of naphthalene. A comparison was made of the effect of three biomass measures: optical density, total cell protein, and living cell number on the calculated rate of naphthalene disappearance. Living cell number was determined by optimizing a fluorescent staining technique and using epifluorescence microscopy. All three techniques gave similar results in terms of the specific growth rates, and in all three there was a lag time between the disappearance of naphthalene and the appearance of biomass. This was most likely a consequence of the production of intermediate products detected in the chromatograms. Inclusion of a one-hour lag time in the integrated Monod expression considerably improved the agreement between experimental and calculated values. However, further improvements to an understanding of the overall rate processes may require more detailed kinetics of the individual biochemical pathways.

Identifier

0035992331 (Scopus)

Publication Title

Water Environment Research

External Full Text Location

https://doi.org/10.2175/106143002x140008

ISSN

10614303

PubMed ID

12150250

First Page

272

Last Page

279

Issue

3

Volume

74

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