PARTition of microchannels with collagen for fabricating tunable 3D cellular microenvironments

Document Type

Conference Proceeding

Publication Date

1-1-2008

Abstract

The ability to model accurately the cellular microenvironment is required to improve studies of cell biology in vitro. Here, we describe a method based on the use of laminar flow that makes it possible to pattern biologically-derived hydrogel (collagen). This technique allows the replication of the cellular microenvironment with control over the distribution of (i) various types of cells and of (ii) gradients of soluble factors, such as cytokines. This technique does not require the use of elements that might be toxic (UV light or photoinitiators) for biological cells. We demonstrate this method by investigating the intercellular communication between two types of macrophage-like cell lines (BAC and LADMAC cells). © 2008 CBMS.

Identifier

84902449636 (Scopus)

Publication Title

12th International Conference on Miniaturized Systems for Chemistry and Life Sciences the Proceedings of Microtas 2008 Conference

First Page

1217

Last Page

1219

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