Probing the membrane potential of living cells by dielectric spectroscopy

Document Type

Article

Publication Date

10-1-2009

Abstract

In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage-sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtained from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli was chosen for our experiments. © 2009 European Biophysical Societies' Association.

Identifier

70349673447 (Scopus)

Publication Title

European Biophysics Journal

External Full Text Location

https://doi.org/10.1007/s00249-009-0507-0

ISSN

01757571

PubMed ID

19579022

First Page

1049

Last Page

1059

Issue

8

Volume

38

Grant

0547427

Fund Ref

National Science Foundation

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