Effect of carbon nanoparticles on renal epithelial cell structure, barrier function, and protein expression

Document Type

Article

Publication Date

9-1-2011

Abstract

To assess effects of carbon nanoparticle (CNP) exposure on renal epithelial cells, fullerenes (C60), single-walled carbon nanotubes (SWNT), and multi-walled carbon nanotubes (MWNT) were incubated with a confluent renal epithelial line for 48 h. At low concentrations, CNP-treated cells exhibited significant decreases in transepithelial electrical resistance (TEER) but no changes in hormone-stimulated ion transport or CNP-induced toxicity or stress responses as measured by lactate dehydrogenase or cytokine release. The changes in TEER, manifested as an inverse relationship with CNP concentration, were mirrored by an inverse correlation between dose and changes in protein expression. Lower, more physiologically relevant, concentrations of CNP have the most profound effects on barrier cell function and protein expression. These results indicate an impact of CNPs on renal epithelial cells at concentrations lower than have been previously studied and suggest caution with regard to increasing CNP levels entering the food chain due to increasing environmental pollution. © 2011 Informa UK, Ltd.

Identifier

80052069092 (Scopus)

Publication Title

Nanotoxicology

External Full Text Location

https://doi.org/10.3109/17435390.2010.514076

e-ISSN

17435404

ISSN

17435390

PubMed ID

21067278

First Page

354

Last Page

371

Issue

3

Volume

5

Grant

R01GM085218

Fund Ref

National Institute of General Medical Sciences

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