Hepatic differentiation from human embryonic stem cells using stromal cells

Document Type

Article

Publication Date

10-1-2011

Abstract

Objective: The derivation of hepatocytes from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the generation of hepatocyte-like cells derived from hES cells. Methods: Hepatic endoderm cells were generated by adding activin A for 5 d- to 1-d-old embryoid bodies formed from hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 feeder cells. Results: After co-culture with mitomycin treated 3T3-J2 feeder cells, these hepatic endodermal cells yielded hepatocyte-like cell colonies, which possessed the proliferation potential to be cultured for an extended period of more than 30 d. With extensive expansion, they co-expressed the hepatic marker AFP and albumin, indicating that they were hepatocyte-like cells. Conclusions: We report the generation of proliferative hepatocyte-like cells from hES cells. These hES cell derived hepatic cells can effectively be used as in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation. © 2011 Elsevier Inc. All rights reserved.

Identifier

80052796039 (Scopus)

Publication Title

Journal of Surgical Research

External Full Text Location

https://doi.org/10.1016/j.jss.2011.06.032

e-ISSN

10958673

ISSN

00224804

PubMed ID

21816427

First Page

e253

Last Page

e261

Issue

2

Volume

170

Grant

ZICNS003008

Fund Ref

National Institute of Neurological Disorders and Stroke

This document is currently not available here.

Share

COinS