Enhanced DNA Detection Through the Incorporation of Nanocones and Cavities into a Plasmonic Grating Sensor Platform

Document Type

Article

Publication Date

5-15-2016

Abstract

In this paper, a novel plasmonic grating sensor platform was developed and tested for feasibility using a lights-ON fluorescence-based DNA assay. The sensor platform combined the fluorescence enhancement of a grating-based plasmonic platform with the electric field intensifying effects of nanoscale cones and cavities. The gratings were made through a microcontact printing process that replicated HD-DVD disks in polymethylsilsesquioxane and coated with a thin gold film. Nanocavities were incorporated into the sensor platform during the printing process and nanocones were incorporated during the 100-nm gold deposition process. Fluorescently tagged single-strand (ss) DNA molecules were immobilized onto the surface and were designed such that the molecules would fluoresce when bound to a complementary sequence. Sensor substrates were imaged after exposure to a mismatched and matched ssDNA to quantify the fluorescence enhancement of the sensor. Exposure to matched ssDNA resulted in fluorescent emission from the grating that was 13.6× brighter than flat gold, while the nanocones and nanocavities were 37.5× and 47.4× brighter than flat gold, respectively.

Identifier

84963820289 (Scopus)

Publication Title

IEEE Sensors Journal

External Full Text Location

https://doi.org/10.1109/JSEN.2015.2479473

ISSN

1530437X

First Page

3403

Last Page

3408

Issue

10

Volume

16

Grant

1102070

Fund Ref

National Science Foundation

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