Author ORCID Identifier

0000-0002-7263-7903

Document Type

Dissertation

Date of Award

12-31-2025

Degree Name

Doctor of Philosophy in Mechanical Engineering - (Ph.D.)

Department

Mechanical and Industrial Engineering

First Advisor

Fatemeh Ahmadpoor

Second Advisor

Dibakar Datta

Third Advisor

Farid Alisafaei

Fourth Advisor

Samaneh Farokhirad

Fifth Advisor

Camelia Prodan

Abstract

Nanoscale structures are inherently dynamic due to persistent thermal fluctuations. Even in solid materials, these random deformations, driven by ambient thermal energy, can become significant when their characteristic scale approaches that of the structure itself and strongly influence their overall mechanical behavior. Examples of such structures include crystalline membranes, appearing in diverse forms such as nanotubes, nanoribbons, and kirigami/origami structures. Similarly, biological nanostructures, including lipid membranes, microtubules, actin filaments, and DNA, exhibit extreme flexibility and responsiveness due to their low bending rigidity. Numerous physiological processes are intrinsically linked to these thermal fluctuations, including exocytosis and endocytosis, membrane fusion, pore formation, cell adhesion, binding and unbinding transitions, self-assembly, vesicle size distributions, red blood cell membrane configurations, and the cytoskeletal or actin mediated mechanics of membranes. These biophysical processes arise from the balance of attractive and repulsive forces between biological structures. Van der Waals forces, which act even between rigid membranes, provide long range attraction, scaling as 1/d3 at short and 1/d6 at larger distances. In flexible membranes, an additional repulsive force known as the entropic force emerges from thermal fluctuations. Entropic force, a long standing topic in biophysics and biomechanics, has been studied for over four decades. Similar to an ideal gas, fluctuating surfaces generate entropic pressure due to thermal fluctuations. Previous studies were mostly limited to fluid, planar, tension free membranes and assumed passive behavior, while real biological membranes often experience activity that enhances their fluctuations. This dissertation offers a comprehensive investigation, addressing key challenges such as surface tension, confinement, membrane size, curvature, nonlinear elasticity, and active forces. Using statistical mechanics, continuum mechanics and computational models for both fluid and solid membranes, we explore how biological factors influence membrane fluctuations and entropic repulsion, with implications for membrane interactions with external objects, nanostructures, viral capsids, and other cells.

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