Document Type


Date of Award


Degree Name

Master of Science in Chemical Engineering - (M.S.)


Chemical Engineering and Chemistry

First Advisor

Hung T. Chen

Second Advisor

Deran Hanesian

Third Advisor

George Y. Lei

Fourth Advisor

John E. McCormick


PH parametric pumping is used to separate protein mixtures of hemoglobin and Albumin.

In this thesis, modifications to a two-column process, where the fluid stage flow pattern and the pH distributions were constant, were explored. These modifications consisted of: changes in the feed stream inlet and outlet locations, changes in the amount of feed itself, and an addition of another column and reservoir to the original two-column process. The results were obtained through computer simulation.

Results indicate that the batch or no-feed two and three column processes yield better separations of the two proteins than the same processes with feed streams. The different feed location cases were ranked and this listing can be found in the results section for the two and three column processes. Also, the conclusion section contains an overall ranking list of both processes.

Results also indicate that the separations obtained for the three-column process were better overall than for the two-column process, and that smaller feed amounts for both processes yielded better separations.



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