A study of human exposure to benzo(a)pyrene (BAP) through different pathways. Part A ; Assessment of benzo(a)pyrene (BAP) exposure through urine analysis with the hydriodic acid reduction reaction. Part B
Date of Award
Master of Science in Environmental Science - (M.S.)
Chemical Engineering, Chemistry and Environmental Science
Trattner, Richard B.
PART A: An investigation of total human exposure to an environmental pollutant is described. The study is being conducted in a community impacted by a foundry suspected to be a dominant local source of benzo(a)pyrene (BaP). The research aims to assess the relative contributions of indoor and outdoor sources and to measure BaP in media including indoor air, outdoor air, food and water.
PART B: A method for biological monitoring of exposure to benzo(a)pyrene, a representative polycyclic aromatic hydrocarbon (PAH), has been developed. The analytical procedure includes extraction of PAH and PAH metabolites from urine using commercial cartridges containing c18-modified silica, reduction of metabolites to PAH ("reverse metabolism") by hydriodic acid, detection by thin layer chromatography (TLC) and plate-scanning spectrofluorometry. This method is derived from a published procedure in which all PAH derived fran PAH-metabolites are analyzed by gas chromatography. The BaP metabolite urine analysis has been applied to:
- A control group of people who voluntarily treated a psoriasis condition with dermal application of coal tar.
- Other human subjects who were more typical of the Phillipsburg population.
Limited studies of the HI reduction of specific metabolites have been performed.
Liang, Shao-Keng, "A study of human exposure to benzo(a)pyrene (BAP) through different pathways. Part A ; Assessment of benzo(a)pyrene (BAP) exposure through urine analysis with the hydriodic acid reduction reaction. Part B" (1988). Theses. 1989.