Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking
Document Type
Article
Publication Date
9-18-2019
Abstract
Mitochondria are essential targets for treatment of diseases with mitochondrial disorders such as diabetes, cancer, and cardiovascular and neurodegenerative diseases. Mitochondria penetrating peptides (MPPs) are composed of cationic and hydrophobic amino acids that can target and permeate the mitochondrial membrane. Herein, a novel d-argine-phenylalanine-d-argine-phenylalanine-d-argine-phenylalanine-NH2 (rFrFrF) was tagged with a rhodamine-based fluorescent chromophore (TAMRA). This probe (TAMRA-rFrFrF) exhibited advantageous properties for long-term mitochondria tracking as demonstrated by fluorescence microscopy. Cell viability assays and oxygen consumption rates indicate low cytotoxicity and high biocompatibility of the new contrast agent. Colocalization studies suggest that TAMRA-rFrFrF is a promising candidate for continuous mitochondrial tracking for up to 3 days.
Identifier
85072311961 (Scopus)
Publication Title
Bioconjugate Chemistry
External Full Text Location
https://doi.org/10.1021/acs.bioconjchem.9b00465
e-ISSN
15204812
ISSN
10431802
PubMed ID
31433175
First Page
2312
Last Page
2316
Issue
9
Volume
30
Grant
1726345
Fund Ref
National Science Foundation
Recommended Citation
Zhao, Tinghan; Liu, Xinglei; Singh, Sweety; Liu, Xiangshan; Zhang, Yuanwei; Sawada, Junko; Komatsu, Masanobu; and Belfield, Kevin D., "Mitochondria Penetrating Peptide-Conjugated TAMRA for Live-Cell Long-Term Tracking" (2019). Faculty Publications. 7336.
https://digitalcommons.njit.edu/fac_pubs/7336
