Vimentin is a key regulator of cell mechanosensing through opposite actions on actomyosin and microtubule networks

Document Type

Article

Publication Date

12-1-2024

Abstract

The cytoskeleton is a complex network of interconnected biopolymers consisting of actin filaments, microtubules, and intermediate filaments. These biopolymers work in concert to transmit cell-generated forces to the extracellular matrix required for cell motility, wound healing, and tissue maintenance. While we know cell-generated forces are driven by actomyosin contractility and balanced by microtubule network resistance, the effect of intermediate filaments on cellular forces is unclear. Using a combination of theoretical modeling and experiments, we show that vimentin intermediate filaments tune cell stress by assisting in both actomyosin-based force transmission and reinforcement of microtubule networks under compression. We show that the competition between these two opposing effects of vimentin is regulated by the microenvironment stiffness. These results reconcile seemingly contradictory results in the literature and provide a unified description of vimentin’s effects on the transmission of cell contractile forces to the extracellular matrix.

Identifier

85194873469 (Scopus)

Publication Title

Communications Biology

External Full Text Location

https://doi.org/10.1038/s42003-024-06366-4

e-ISSN

23993642

PubMed ID

38811770

Issue

1

Volume

7

Grant

DMS-1953572

Fund Ref

National Science Foundation

This document is currently not available here.

Share

COinS