Insights into the roles of biochar pores toward alleviating antibiotic resistance genes accumulation in biofiltration systems

Document Type

Article

Publication Date

2-1-2024

Abstract

Biofiltration systems would harbor and spread various antibiotic resistance genes (ARGs) when treating antibiotic micro-pollution, constituting a potential ecological risk. This study aimed to investigate the effects of biochar pores on ARG emergence and related microbial response mechanisms in bench-scale biofiltration systems. Results showed that biochar pores effectively reduced the absolute copies of the corresponding ARGs sul1 and sul2 by 54.1% by lowering the sorbed-SMX's bioavailability compared to non-porous anthracite. An investigation of antimicrobial resistomes revealed a considerable decrease in the abundance and diversity of ARGs and mobile gene elements. Metagenomic and metaproteomic analysis demonstrated that biochar pores induced the changeover of microbial defense strategy against SMX from blocking SMX uptake by EPS absorbing to SMX biotransformation. Microbial SOS response, antibiotic efflux pump, EPS secretion, and biofilm formation were decreased. Functions related to SMX biotransformation, such as sadABC-mediated transformation, xenobiotics degradation, and metabolism, were significantly promoted.

Identifier

85181815872 (Scopus)

Publication Title

Bioresource Technology

External Full Text Location

https://doi.org/10.1016/j.biortech.2023.130257

e-ISSN

18732976

ISSN

09608524

PubMed ID

38151208

Volume

394

Grant

CBET - 1903597

Fund Ref

National Science Foundation

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