Assessment of the efficiency of DNA isolation and profiling applying a temperature-driven method in human remains

Document Type

Article

Publication Date

5-1-2024

Abstract

The identification of human remains is of utmost importance in a variety of scenarios. One of the primary identification methods is DNA. DNA extraction from human remains could be difficult, particularly in situations where the remains have been exposed to environmental conditions and other insults. Several studies tried to improve extraction by applying different approaches. ForensicGEM Universal (MicroGem) is a single-tube approach to DNA extraction and a temperature-driven method that could have some advantages with respect to previous techniques, among them, reducing the risk of contamination, not requiring specialized equipment, or several steps to perform. The aim of this study was to assess, for the first time, the efficiency of DNA extraction and quality of STR profiles applying the MicroGem protocol and modifications of this protocol from tooth samples in comparison with automatic extraction (AE). Our results indicated that AE and MicroGem performed similar, though with variability depending on the MicroGem modifications, increasing the DNA yield and STR profile quality when DNA is concentrated with Microcon. These findings demonstrated the efficiency of this methodology for DNA extraction from human remains while also providing a simple and quick technique suitable to apply in a variety of forensic scenarios.

Identifier

85182808471 (Scopus)

Publication Title

Electrophoresis

External Full Text Location

https://doi.org/10.1002/elps.202300273

e-ISSN

15222683

ISSN

01730835

PubMed ID

38247192

First Page

805

Last Page

813

Issue

9-10

Volume

45

Fund Ref

Universidad de Málaga

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