3D Bioprinted Hydrogel Microfluidic Devices for Parallel Drug Screening

Document Type

Article

Publication Date

1-1-2022

Abstract

Conventional high-throughput screening (HTS) platforms suffer from the need for large cell volumes, high reagent consumption, significant assembly cost, and handling efforts. The assembly of three-dimensional (3D) bioprinted hydrogel-based microfluidic chips within platforms can address these problems. We present a continuous and seamless manufacturing approach to create a bioprinted microfluidic chips with a circular pattern scalable toward HTS platforms. Digital light processing 3D bioprinting is used to tune the local permeability of our chip, made of polyethylene glycol diacrylate and cell-laden gelatin methacryloyl, for creating predefined gradients of biochemical properties. We measured the flow-induced physical characteristics, the mass transport of drug agents, and the biological features of the proposed chip. We measured reactive oxygen species from the encapsulated cells through an integrated process and showed the capacity of the hydrogel-based chip for creating drug/agent gradients. This work introduces a chip design based on a hydrogel that can be changed and could be used for modern HTS platforms such as in vitro organoids.

Identifier

85137634612 (Scopus)

Publication Title

ACS Applied Bio Materials

External Full Text Location

https://doi.org/10.1021/acsabm.2c00578

e-ISSN

25766422

First Page

4480

Last Page

4492

Issue

9

Volume

5

Fund Ref

Rowan University

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