Utilization of flow cytometry in nanomaterial/bionanomaterial detection

Document Type

Syllabus

Publication Date

1-1-2022

Abstract

Nanomaterials have become inevitable components in various sectors of life and commerce. Maximum utilization of nanomaterials in chemical, pharmaceutical, and cosmetic industries, albeit loaded with abundant biological activities and advantageous alike, leads to toxicity in humans and environment. The nanomaterials penetrate into tissues, porous structures, and membranes and tend to invade and remain within cells and body fluids to eventually cause harmful effects to humans and other mammals. In order to identify and shun the nanomaterials from the body, they must be detected, characterized, and quantified, which require high-end technologies. Flow cytometry is one advanced technique generally used for learning the characteristics of selected populations of prokaryotic and eukaryotic cells. This technique also aids in detecting and delivering information on physicochemical features of the nanomaterials present in the fluid suspensions. In this chapter, an elaborate discussion would be carried out on the techniques involved in investigating and analyzing the nanomaterials using flow cytometer with laser radiations. The mechanisms of the working of flow cytometer in detection of nanomaterials would be highlighted here. A significant study would be performed on side scattered light signal to examine the residing nanomaterials within cellular structures. This chapter shall be beneficial in exposing the potential utilization of flow cytometry in measuring the nanomaterial uptake by the cells and determining human health.

Identifier

85137944625 (Scopus)

ISBN

[9780128235935, 9780128234266]

Publication Title

Handbook of Microbial Nanotechnology

External Full Text Location

https://doi.org/10.1016/B978-0-12-823426-6.00016-4

First Page

133

Last Page

144

This document is currently not available here.

Share

COinS